Description

XL1-Blue MRF’ and XL1-Blue MR competent cells allow representational cloning of methylated DNA. Transformation efficiency for XL1-Blue MR and XL-1 Blue MRF’ supercompetent cells of =1 x 10^9 transformants/µg Transformation efficiency for XL1-Blue MRF´ Kan supercompetent cells of > 1 x 10^9 transformants/µg Transformation efficiency for XL1-Blue MRF’ electroporation competent cells of > 1 x 10^10 transformants/µg XL-1 Blue MRF’ and XL-1 Blue MR competent cells allow high efficiency and representational cloning of methylated DNA. All known E. coli K12 restriction systems have been deleted. Use XL1-Blue MRF’ cells when cloning methylated cDNA or genomic DNA, or when cloning methylated PCR products. When the F’ episome and blue-white screening are unnecessary, use XL1-Blue MR supercompetent cells. When cloning tetracycline-resistant plasmids, use XL1-Blue MRF’ Kan supercompetent cells to select for the F’ episome and provide a more intense blue color for blue-white screening. This product is only available to U.S. Domestic Customers. For research use only, not to be used in diagnostic procedures.