Description

PathDetect cis-reporting systems assess in vivo activation of signal transduction pathways. Identify pathway-specific transcription activation Easier and faster than blotting or gel-shift assays Sensitive, quantitative assays Determine if a gene product or compound activates pathways leading to specific enhancers with our PathDetect cis-reporting systems. When PathDetect cis-reporting and expression plasmids encoding your gene of interest are cotransfected into mammalian cells, increased reporter activity indicates transcription activation and involvement of your gene product in the pathway leading to the enhancer element. For ultimate flexibility, our pLuc-MCS expression vector includes multiple cloning sites to facilitate insertion of your enhancer or transcription element. The system also includes positive and negative controls that ensure the success of your experiments. This product is only available to U.S. Domestic Customers. For research use only, not to be used in diagnostic procedures.