Description

The innuSPEED Stool DNA Kit provides an effective means of extracting microbial DNA from stool samples. The first step in the process is homogenization (e.g. with a SpeedMill) of the solid or liquid samples in a Lysis Tube using rapidly accelerated beads. This step is then followed by thermal lysis. This highly efficient homogenization process is followed by a prefiltration step, which serves as an optimum method for removing undissolved sample components. Microbial DNA are then selectively bound to the surface of a Spin Filter membrane; this is followed first by wash steps to remove inhibitors, and then by addition of a low-salt buffer to separate bound DNA from the membrane. The DNA is then ready for use in all subsequent applications. Features Isolation of bacterial DNA from solid or liquid stool samples Includes prefiltration for removing undissolved sample components Lysis Tubes and adapted beads for optimum sample homogenization Suitable for use with homogenizers (such as the SpeedMill or others)