Description

Achieve consistent high specificity during qRT-PCR detection of miRNA in real time Achieve accurate real-time PCR quantification for miRNA. A high-specificity reagent system that delivers consistent, sensitive and reproducible results. The kit elongates miRNAs in a polyadenylation reaction and then reverse transcribes the polyadenylated RNA into QPCR-ready cDNA. The reagents included enable the synthesis of first-strand cDNA from the tailed miRNAs. The kit master mix contains all of the necessary enzymes, dyes (including EvaGreen® Dye) and reagents to carry out accurate qRT-PCR detection of microRNA. Our miRNA qRT-PCR detection system enables accurate and real-time PCR quantification for miRNA. The kit is a high-specificity reagent system that delivers sensitive and reproducible results. The kit elongates miRNAs in a polyadenylation reaction and then reverse transcribes the polyadenylated RNA into QPCR-ready cDNA. ;The reagents provided enable the synthesis of first-strand cDNA from the tailed miRNAs. In addition, the master mix contains all of the necessary enzymes, dyes (including EvaGreen Dye), and reagents to carry out accurate qRT-PCR detection of microRNA. This product is only available to U.S. Domestic Customers. For research use only, not to be used in diagnostic procedures.