Description

Provides the highest efficiency of conversion of RNA to cDNA, fully optimized for qPCR applications. Optimized for real-time quantitative PCR (qPCR) application;Produces cDNA in 15 minutes with linear detection from 3 pg to 3 µg total RNA;Creates up to 12 kb cDNA in 25 minutes or less;Available as a first-strand synthesis kit and a two-step QRT-PCR master mix format The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.;The streamlined protocol produces cDNA in 15 minutes with linear detection from 3 pg to 3 µg of total RNA. The easy-to-use process generates up to 12 kb cDNA in 25 minutes or less. Available as a first-strand synthesis kit and a two-step QRT-PCR master mix format, using SYBR Green or probe-based detection. This product is only available to U.S. Domestic Customers. For research use only, not to be used in diagnostic procedures.