Description

Agilent Endoglycosidases (formerly ProZyme) release oligosaccharides from glycoproteins and some other substrates. These enzymes are useful for deglycosylating glycoproteins prior to analysis, or for releasing N-glycans for labeling and analysis. Useful in N-glycan analysis, Agilent offers PNGase F, an asparagine amidase that releases intact N-glycans. PNGase F cleaves between the innermost GlcNac of the N-glycan and the asparagine (Asn) residue of the peptide to which the N-glycan is linked. AdvanceBio N-Glycanase-ultra (PNGase F, EDTA free), =10 U/mL (formerly ProZyme). A recombinant form of PNGase F (400 mU, EDTA free), releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. The same formulation provided in GlykoPrep, = 10 U/mL and EDTA-free. Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution. This product is only available to U.S. Domestic Customers. For research use only, not to be used in diagnostic procedures.