Description

Agilent Endoglycosidases (formerly ProZyme) release oligosaccharides from glycoproteins and some other substrates. These enzymes are useful for deglycosylating glycoproteins prior to analysis, or for releasing N-glycans for labeling and analysis. Useful in N-glycan analysis, Agilent offers PNGase F, an asparagine amidase that releases intact N-glycans. PNGase F cleaves between the innermost GlcNac of the N-glycan and the asparagine (Asn) residue of the peptide to which the N-glycan is linked. AdvanceBio N-Glycanase-plus (PNGase F), =10 U/mL (formerly ProZyme). Enzyme releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. A recombinant form of PNGase F. Includes 5x N-glycanase reaction buffer:100 mM sodium phosphate pH 7.5, 0.1% sodium azide; denaturation solution: 2% SDS, 1 M 2-mercaptoethanol; detergent solution: 15% detergent; 5x N-glycanase Tris reaction buffer: 50 mM Tris-HCl pH 8.0. This product is only available to U.S. Domestic Customers. For research use only, not to be used in diagnostic procedures.