Description

The innuSPEED Bacteria/Fungi RNA Kit has been designed for rapid homogenization and includes an efficient process for digesting the cell walls of fungal spores and gram+ bacteria in specially developed Lysis Tubes; the process utilizes the SpeedMill (or other homogenizers). The starting sample is then denatured; the unique Lysis Buffer deactivates endogenous and exogenous RNases. Once the genomic DNA has been selectively removed, the RNA is bound to the surfaceof a Spin Filter membrane, washed and then finally eluted from the membrane. The isolated RNA is immediately available for downstream applications. Features Extraction of total cellular RNA from fungal spores and gram+ bacteria No DNase I digestion: filter membrane for fast, selective removal of genomic DNA Integrated inactivation of endogenous and exogenous RNases Improved extraction without the use of toxic β-mercaptoethanol