Description

Rugged, high and low pH separations of polypeptides and peptides from pH 2-11.5 Different selectivity possible at high and low pH High efficiency and good recovery of hydrophobic peptides at high pH Ideal for GC/MS with ammonium hydroxide-modified mobile phase ZORBAX 300Å Extend C-18 is a wide-pore HPLC column for high efficiency separations of peptides from pH 2-11.5. The unique, bidentate bonded phase provides excellent lifetime and reproducibility at high and low pH. At high pH, retention and selectivity of peptides and polypeptides can change dramatically as a result of changes in charge on molecules. Excellent recoveries of hydrophobic polypeptides have been achieved at room temperature and high pH. GC/MS sensitivity of peptides and polypeptides can also be improved at high pH using a simple ammonium hydroxide-containing mobile phase. *RR: Rapid Resolution 3.5 µm Agilent Technologies products are intended for sale/shipment within the US only. International customers should contact their local Agilent Technologies distributor to place orders for Agilent Technologies products.