Description

A DNA-dependent RNA polymerase with high sequence specificity for SP6 promoter sequences Probes for nucleic acid hybridization Templates for in vitro translation Substrates for RNA processing studies Exon and intron mapping of genomic DNA SP6 RNA Polymerase is a DNA-dependent RNA polymerase with high sequence specificity for SP6 promoter sequences. SP6 RNA polymerase synthesizes RNA 5' to 3' and incorporates  35 S, 32 P and  33 P ribonucleotides. This enzyme is isolated from an overproducing recombinant E. coli clone. SDS-polyacrylamide gel electrophoresis analysis of the SP6 RNA Polymerase assures 99% purity. Unit definition: One unit will catalyze the incorporation of 1 nmole of nucleoside triphosphate into an acid insoluble form in 60 minutes at 37°C. Reaction Conditions: 40 mM Tris-HCl (pH 8.0), 8 mM MgCl2, 50 mM NaCl, 2 mM spermidine, 30 mM DTT, 400 µM of each rNTP, 1 µg DNA template and 20 units enzyme in a 25-µl volume. Incubate 60 minutes at 37°C. This product is only available to U.S. Domestic Customers. For research use only, not to be used in diagnostic procedures.