Description

Mammalian cloning and expression vector Contains pMC1neo Vector, pMC1neo Poly A Vector, and AG1 Strain (bacterial glycerol stock). Applications: Use the pMC1neo vector when cloned fragment provides poly(A) adenylation signal for neomycin gene, and use the pMC1neo Poly A vector when polyadenylation signal is not present. Cloning Sites: Unique Sal I and Xho I sites for cloning sequences of interest. Selection: E. coli: ampicillin resistant, kanamycin sensitive Eukaryotic cells: G418 resistance Homologous Recombination: The G418-resistance cartridge is introduced by homologous recombination between the cloned sequence of interest and its genomic copy following transfection of cells. Cells that have undergone homologous recombination are G418 resistant. In a model system, 1/1000 cells that were G418-resistant had also undergone a gene targeting event. Agilent cloning vector which includes pMC1neo Vector, pMC1neo Poly A Vector, and AG1 Strain (bacterial glycerol stock). This product is only available to U.S. Domestic Customers. For research use only, not to be used in diagnostic procedures.