Description

Agilent’s 1-10 kb DNA ladder is a premixed, ready-to-load molecular weight marker with fragments from 1 to 10 kb. Fragments are exact multiples or fractions of 1000 bp Includes visual reference point for determining location More economical than competitive markers Easy radiolabeling of ladder Known mass amount of each fragment for DNA quantitation The DNA Ladder contains fragments from 2 to 10 kb at 1 kb increments and an additional higher band at 12 kb as well as bands at 250, 500, 750 and 1500 bp. The higher bands are brighter than those at the lower end, with the transition to higher intensity at the 2 kb band serving as a useful reference point. Each fragment contains a 5' overhang (TTAA) that can be radioactively labeled using the Klenow Fill-In Kit. DNA fragments may also be detected via nonradioactive Southern blot analysis by incorporating fluor-modified nucleotides into the DNA prior to electrophoresis and using a tan Illuminator Chemiluminescent Detection System. This product is only available to U.S. Domestic Customers. For research use only, not to be used in diagnostic procedures.