Description

AAV-293 cells are an HEK293 cell line optimized for packaging of AAV virions in helper-free systems. AAV-293 cells are derived from the commonly used HEK293 cell line but produce higher viral titers. HEK293 cells are human embryonic kidney cells transformed by sheared adenovirus type 5 DNA. Replication of the AAV-2 vector requires the adenovirus E1 gene product stably expressed in the HEK293-derived AAV-293 cells. AAV-293 cells are an HEK293 cell line optimized for packaging of AAV virions in the helper-free system. AAV-293 cells, like HEK293 cells, produce E1 in trans. AAV-293 cells allow production of infectious virus particles when transfected with E1-deleted adenovirus vectors or co-transfected with the three AAV helper-free system plasmids. These plasmids supply all of the trans-acting factors required for AAV replication and packaging in AAV-293 cells. Recombinant AAV-2 viral particles prepared from infected AAV-293 cells may then be used to infect a variety of mammalian cells. This product is only available to U.S. Domestic Customers. For research use only, not to be used in diagnostic procedures.